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Scalability

Thomson Optimum Growth™ Flasks are designed so that protein production will scale consistently across all sizes, unlike any other shake flasks on the market. Additionally, these flask features allow for consistent shake speeds from the 125mL flasks up to the 5L flasks.

Space Saving More Volume

Optimum Growth™ Flasks give excellent growth with space saving capability. For more information see our Optimum Growth™ accessories page

fig. 1. 18 x 1.6L Optimum Growth™ Flasks Total Volume 16.2L/Shaker

fig. 2. 6 x Corning® 3L Total Volume 6L/Shaker

fig. 3. 8 Position Carrier for 125mL & 250mL flasks

fig. 4. 12 x Optimum Growth™ 2.8L Flasks Total Volume 16.8L/Shaker

Fill Volumes & Shake Speeds

CHO Stable Cells, CHO Transient, HEK293 Transient , Hybridoma

Insect Cells

Microbes/E. coli

Data provided by Genentech part of the Roche Group

Corning® vs Optimum Growth™ Flasks: Comparison of Two Expressed Membrane Proteins

• Corning® – 500mL flask, 200mL culture
• Thomson – 250mL flask, 150mL culture
• 4mL samples purified over Ni-NTA
• Protein A – Membrane protein of moderate expression, 34kDa
• Protein B – Membrane protein of low expression, 45kDa
• 12µL of elution resolved on a coomassie gel

Conclusion: Thomson flasks work at least as good as Corning® standard. Improved working volume/flask volume ratio when using Thomson.

214% Yield Increase From Insect Cells

Protein Production/Flask

Data supplied by New York Structural Genomics Researsh Consortium

Same Footprint-Double Volume

Optimum Growth™ 5L (3L Media)
vs Nalgene® Nunc 2.8L (1.5L Media)

High & Low Expressing Proteins in HEK293 Cells

4-20% SDS-PAGE Quick Blue Stain Commassie Gel
Expected MW of dimer 24.5 kDa Estimated expression level ~10-20 mg/L

Low Expressing Gel

This gel shows equal bands from 5 replicates of a low expressing protein, producing roughly 10 to 20 mg/L.

1. Benchmark Pre-Stained Protein Ladder
2. Purified protein, 100 ng control
3. Purified protein, 200ng control
4. Untransfected cells, -ve control
5. +ve control
6. +ve control
7. Protein of interest, 5L Combined Flasks #1-5
8. Protein of interest, 5L Flask #1
9. Protein of interest, 5L Flask #2
10. Protein of interest, 5L Flask #3
11. Protein of interest, 5L Flask #4
12. Protein of interest, 5L Flask #5

4-20% SDS-PAGE Quick Blue Stain Commassie Gel
Expected MW of dimer 159.4 kDa Estimated expression level ~300 mg/L

High Expressing Gel

Thomson 5L flasks are consistently able to maximize production of your best expressers. This gel shows equal bands from 3 replicates of a high expressing protein, producing approximately 300 mg/L.

1. Benchmark Pre-Stained Protein Ladder
2. Purified mAb 100 ng control
3. Purified mAb 250 ng control
4. Purified mAb 500 ng control
5. Purified mAb 1000 ng control
6. +ve control
7. Protein of interest, 5L Flask #1
8. Protein of interest, 5L Flask #2
9. Protein of interest, 5L Flask #3
10. Protein of interest, 5L Combined Flasks #1-3

Conclusion

Thomson Optimum Growth™ Flasks not only ensure consistent expression from HEK293 strains, they can also increase shaker capacity. With the same footprint as a typical Corning® 3L flask and a culture volume of up to 3L, the Optimum Growth™ 5L Flask may increase production 200%, if not more, in the same space (this is construct dependent).

Most constructs express at higher levels in the Optimum Growth™ 5L flasks. This makes one Optimum Growth™ 5L equivalent to, if not greater than, two 3L flasks.

Thomson Instrument Company is not affiliated with Corning® Life Sciences or their products

Flask Clamp Compatibility

Use the chart below to find the appropriate flask clamp compatible with your shaker. https://htslabs.com/og/