Plasmid Purification-Kits High-Speed / Fast Ion
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.
- Specifications Mini Kit // Midi Kit
- Method: Spin column // Anion-Exchange
- Sample size: 1 – 5 ml // 50 ml high-copy plasmid / 100 ml low-copy plasmid
- Binding capacity: 30 µg // 800 µg
- Fragment size: 1 – 15 kb // 1 – 20 kb
- Typical yield: 10- 30 µg // 200 – 800 µg
- Operation Time: <15 min. // <120 min.
Toote variatsioonid
| Catalog number | PK | Binding capacity | Fragment size | Max. volume sample | Min. volume sample | Number of test fields | Preparation time | Product specific notes | Type | Volume sample | Yield | Kogus | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4.661 763 | 1.00 | 800 µg | 1 ... 20 kb | 300 ml | 50 ml | 25 | 120 min | Anion-Exchange, gravity flow | MIDI Fast Ion | 50 ... 300 ml | 200 ... 800 µg | ||
| 4.661 765 | 1.00 | 30 µg | 1 ... 15 kb | 5 ml | 1 ml | 100 | 15 min | Spin column | Hi-Speed MINI | 1 ... 5 ml | 10 ... 30 µg | ||
| 4.661 767 | 1.00 | 30 µg | 1 ... 15 kb | 5 ml | 1 ml | 300 | 15 min | Spin column | Hi-Speed MINI | 1 ... 5 ml | 10 ... 30 µg |
