Plasmid Purification-Kits High-Speed / Fast Ion
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.
- Specifications Mini Kit // Midi Kit
- Method: Spin column // Anion-Exchange
- Sample size: 1 – 5 ml // 50 ml high-copy plasmid / 100 ml low-copy plasmid
- Binding capacity: 30 µg // 800 µg
- Fragment size: 1 – 15 kb // 1 – 20 kb
- Typical yield: 10- 30 µg // 200 – 800 µg
- Operation Time: <15 min. // <120 min.
Toote variatsioonid
| Type | Description | For | Catalog number | PK | Kogus | |
|---|---|---|---|---|---|---|
| Hi-Speed MINI | Spin column | 300 preps | 4.661 767 | 1.00 | ||
| Hi-Speed MINI | Spin column | 100 preps | 4.661 765 | 1.00 | ||
| MIDI Fast Ion | Gravity flow | 25 preps | 4.661 763 | 1.00 |
