Plasmid Purification-Kits I-Blue Mini / Midi

For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.

• Fast extraction time
• High yield
• Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator

• Specifications I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit
• Method: Spin column // Anion-Exchange
• Sample size: 1 – 7 ml // 50 – 100 ml high-copy plasmid / 100 – 150 ml low-copy plasmid
• Binding capacity: 50 µg // 500 µg
• Fragment size: 1 – 15 kb // 1 – 20 kb
• Typical yield: up to 50 µg // 200 – 500 µg
• Operation Time: <15 min. // <80 min.